Thermo Fisher Scientific Pepsin A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PGA4(Accession P0DJD7), corresponding to amino acid residues N352-A388. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Pepsin A,uniProtId:P0DJD7-1,ncbiNodeId:9606,antigenRange:352-388,antigenLength:388,antigenImageFileName:PA5-103156_Pepsin_A_P0DJD7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-103156_Pepsin_A_P0DJD7-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Pepsin A,uniProtId:P0DJD8-1,ncbiNodeId:9606,antigenRange:388,antigenLength:388,antigenImageFileName:PA5-103156_Pepsin_A_P0DJD8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-103156_Pepsin_A_P0DJD8-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Pepsin A,uniProtId:P0DJD9-1,ncbiNodeId:9606,antigenRange:388,antigenLength:388,antigenImageFileName:PA5-103156_Pepsin_A_P0DJD9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-103156_Pepsin_A_P0DJD9-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2852526

Product Specific Information

Antibody detects endogenous levels of total Pepsin A.

Target Information

Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Pepsin A is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. Pepsin A is inhibited by ovUS-1, a uterine serpin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.