Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (1.0 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 506, FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

Description:
The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). CD11b is expressed on monocytes, granulocytes, activated lymphocytes, and some NK cells, acting as a receptor for ICAM family members and iC3b, crucial for cell-cell and cell-matrix interactions.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood. Use 5 µL (1.0 µg) per 100 µL test volume. Cell count typically 10⁵–10⁸ cells/test.

Fluorophore Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm; optimized for ultraviolet (355 nm) laser-equipped cytometers. Verify instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light; dye is sensitive to photo-induced oxidation.

Fixation:
Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333). Compatible with short-term methanol-based fixation (<1 hour).

Laser Information:

• Excitation: 355 nm
• Emission: 732 nm
• Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) forms a heterodimer with integrin beta-2 (CD18), known as Mac-1 or CR3. Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
CD11b is a key marker for microglia and macrophage subsets, with expression levels varying by tissue and maturation stage. Dysregulation is linked to systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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