Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), Brilliant Ultra Violet 737, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• eFluor 450
• eFluor 506
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c associates with beta 2 integrin to form the CD11c/CD18 heterodimer, expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. This complex binds CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells; use 5 µL (0.5 µg) per test (final volume 100 µL, 10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, for use on cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendation:
Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.

Methanol Compatibility:
Short-term methanol fixation is compatible; avoid storage in methanol-containing buffers longer than one hour.

Optical Properties:
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

CD11, together with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed on B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed on monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed on monocytes, macrophages, NK cells, granulocytes, and subsets of lymphocytes

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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