Thermo Fisher Scientific N-terminal Arginylation Monoclonal Antibody (4A9), FITC
N-terminal 아르기닐화 단백질 검출용 FITC 결합 단일클론 항체. Western blot과 유세포분석에 적합. N-terminal 아르기닌-글루탐산 특이적 결합. 단백질 G 정제, 보존제 없음, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A9 |
| Immunogen | Synthetic N-terminal arginylated peptide conjugated to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.4) with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931825 |
Available Formats
- Unconjugated (MA5-27563)
- APC (MA5-45370)
- PE (MA5-45373)
- PerCP (MA5-45372)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45371 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
Target Information
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. It also regulates cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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