Thermo Fisher Scientific N-terminal Arginylation Monoclonal Antibody (4A9), APC
N-terminal Arginylation을 특이적으로 인식하는 마우스 단클론 항체로, APC 형광 표지. Western blot 및 유세포분석에 최적화. 고순도의 Protein G 정제 항체로 보존제 없음. 연구용으로 단백질 아르지닐화 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A9 |
| Immunogen | Synthetic N-terminal arginylated peptide conjugated to KLH |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931824 |
Additional Formats
- Unconjugated (MA5-27563)
- FITC (MA5-45371)
- PE (MA5-45373)
- PerCP (MA5-45372)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45370 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
Target Information
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. It also regulates cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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