Thermo Fisher Scientific N-terminal Arginylation Monoclonal Antibody (4A9), PerCP
N-terminal arginylation을 특이적으로 검출하는 마우스 모노클로날 항체로 PerCP 형광 표지. Western blot 및 유세포분석(Flow cytometry)에 최적화. 높은 특이성과 재현성을 제공하며 연구용으로 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A9 |
| Immunogen | Synthetic N-terminal arginylated peptide conjugated to KLH |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931826 |
Available Formats
Product Specific Information
A 1:1,000 dilution of MA5-45372 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
Target Information
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. It also regulates cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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