Thermo Fisher Scientific NTF3 Polyclonal Antibody, PeproTech
Goat 유래의 Polyclonal 항체로 Human NTF3 단백질을 특이적으로 인식합니다. Western blot, ELISA, 중화 실험에 사용 가능합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P82G-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NTF3 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
| Neutralization | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human NT-3 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930791 |
Product Specific Information
AA Sequence of recombinant protein:
MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human NT-3. Anti-Human NT-3-specific antibody was purified by affinity chromatography employing an immobilized Human NT-3 matrix.
Sandwich ELISA:
To detect Human NT-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NT-3 (500-P82GBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human NT-3.
Western Blot:
To detect Human NT-3 by Western Blot analysis, this antibody can be used at 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human NT-3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. It is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system and affect neuronal development in the embryo when expressed in human placenta.
NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined, including human, pig, rat, and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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