Thermo Fisher Scientific NTF4 Polyclonal Antibody, PeproTech
Rabbit 유래의 Polyclonal 항체로 Human NTF4 단백질을 검출. Western blot 및 ELISA에 최적화된 고순도 항체. Affinity chromatography로 정제되었으며, PBS buffer에 동결건조 형태로 제공. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P83-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NTF4 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 28.1 kDa Recombinant Human NT-4 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2942233 |
Product Specific Information
Amino Acid Sequence (recombinant protein, monomer):
GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NT-4. The anti-Human NT-4-specific antibody was purified by affinity chromatography employing an immobilized Human NT-4 matrix.
Sandwich ELISA:
To detect Human NT-4 by sandwich ELISA (100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Human NT-4 (500-P83BT) as a detection antibody, it enables detection of at least 2000–4000 pg/mL of Recombinant Human NT-4.
Western Blot:
For Human NT-4 detection by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human NT-4 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
This gene belongs to the neurotrophin family, which regulates survival and differentiation of mammalian neurons. Expression is ubiquitous and less influenced by environmental signals. Unlike other neurotrophins (NGF, BDNF, NT-3), NTF5-deficient mice show minor cellular deficits and develop normally to adulthood.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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