Thermo Fisher Scientific hnRNP K Polyclonal Antibody
Thermo Fisher Scientific의 hnRNP K Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 Western blot, IHC, ICC/IF, RIP 등 다양한 응용에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 안정적인 장기 보관이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| RNA Immunoprecipitation (RIP) | 1.25 µg/10^6 cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–196 of Human hnRNP K |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.18 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544998 |
Product Specific Information
- Recommended positive controls: 293T, A431, Hela, HepG2, Mouse brain
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (81%), Dog (98%), Pig (99%), Rabbit (99%), Rhesus Monkey (100%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
While all hnRNPs are present in the nucleus, some shuttle between the nucleus and cytoplasm. hnRNP K is located in the nucleoplasm and contains three KH domains that bind RNAs, showing strong affinity for poly(C). It may also play a role in cell cycle progression. Several alternatively spliced transcript variants have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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