Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), APC, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 0.06 µg/test
• Publications: View 44 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-30S
• NovaFluor Red 685 / 710
• NovaFluor Yellow 570 / 590 / 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA).
This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells.
CD24 is variably glycosylated, resulting in heterogeneity of molecular mass across cell types. Antibodies to CD24 show subtle differences in staining intensity among lymphocyte populations.

Expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes
• Recommended Usage: ≤0.06 µg/test (defined as the amount to stain cells in 100 µL final volume)
• Cell Number Range: 10⁵–10⁸ cells/test (empirically determined)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells to mature surface Ig-positive B cells.
Plasma cell expression is very low or absent.
CD24 is also expressed on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas.
It may play a role in regulating B-cell proliferation, maturation, and autoimmunity control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지