Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), APC, eBioscience
마우스 CD24 항원을 인식하는 M1/69 단클론 항체로, APC 형광체가 결합된 형광세포분석용 시약입니다. 0.06 µg/test 농도로 사용 가능하며, 633–647 nm에서 여기되어 660 nm에서 방출됩니다. B세포 발달 단계 분석 및 면역세포 표지에 적합합니다.
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Applications
- Tested Application: Flow Cytometry (Flow)
- Tested Dilution: 0.06 µg/test
- Publications: View 44 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/69 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852841 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-30S
- NovaFluor Red 685 / 710
- NovaFluor Yellow 570 / 590 / 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Request custom conjugation
Product Specific Information
The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA).
This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells.
CD24 is variably glycosylated, resulting in heterogeneity of molecular mass across cell types. Antibodies to CD24 show subtle differences in staining intensity among lymphocyte populations.
Expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes
- Recommended Usage: ≤0.06 µg/test (defined as the amount to stain cells in 100 µL final volume)
- Cell Number Range: 10⁵–10⁸ cells/test (empirically determined)
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells to mature surface Ig-positive B cells.
Plasma cell expression is very low or absent.
CD24 is also expressed on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas.
It may play a role in regulating B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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