Thermo Fisher Scientific ERK1/2 Recombinant Mouse Monoclonal Antibody (MILAN8R)

Applications

• Western Blot (WB)

Tested Dilution: 1:1,000
Publications: - References

Product Specifications

Additional Formats:

• HRP
• Request custom conjugation

Product Specific Information

This MILAN8R monoclonal antibody recognizes human and mouse extracellular signal-regulated kinases 1 and 2 (ERK1/2, p44/p42, MAPK3/1) when phosphorylated on T202/Y204.

Recombinant mouse monoclonal antibodies are produced using in vitro expression systems from specific genes coding for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro.

Advantages of recombinant antibodies:

• Enhanced specificity
• Lot-to-lot consistency

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

ERK1 and ERK2 are widely expressed and play roles in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. They are activated by various stimuli such as growth factors, cytokines, viral infection, GPCR ligands, and transforming agents.

Upon activation, Ras interacts with Raf, which phosphorylates MEK1/2. MEK1/2 then phosphorylates ERK1/2 on tyrosine/threonine residues. Activated ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.