Thermo Fisher Scientific Human TGF-beta 3 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human TGF-beta 3 recombinant protein is a homodimer containing two 112 amino acid subunits

Molecular weight

25.4 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE and HPLC

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 ≤ 0.05 ng/mL (≥ 12.5x10e6 IU/mg); determined by a toxicity assay using the WHO Standard 98/608 for direct comparison.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

0.12% acetic acid with 20% ethanol

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

•Purity: >98% by SDS-PAGE and HPLC
•Endotoxin Concentration: <0.1 ng/µg
•Protein Length: 112 aa (2 identical subunits)
•Biological Activity: ED50 ≤0.05 ng/mL
•Molecular Weight: 17.5 kDa

Target Information

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.