Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Phospho-Histone H3 (Ser10) 폴리클로날 항체로 세포 내 히스톤 H3의 Ser10 인산화 상태를 검출. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제 및 안정적 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated synthetic peptide within amino acid region 1–50 on HH3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes multiple post-translational modifications such as methylation, acetylation, and phosphorylation, influencing diverse cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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