
Thermo Fisher Scientific CHEK2 Monoclonal Antibody (OTI5C7), TrueMAB
CHEK2 단백질을 인식하는 Mouse Monoclonal Antibody로 Western Blot, IHC, Flow Cytometry, IP에 적합. Human 및 Dog 반응성. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. DNA 손상 반응 및 암 관련 연구에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C7 |
| Immunogen | Full-length human recombinant CHEK2 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with cancer, including Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.
CHK2 is activated by DNA damage and phosphorylates several cell cycle regulators, including tumor suppressor proteins such as BRCA1, contributing to cellular survival after DNA damage.
It functions as a putative tumor suppressor protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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