Takara Oxidation-resistant recombinant RNase inhibitor
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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 |
2315A | Takara 2315A Recombinant RNase Inhibitor ver.2.0, 5,000 U pk | 재고문의 | pk | 131,000원 | 144,100원 | |
2315B | Takara 2315B Recombinant RNase Inhibitor ver.2.0, 25,000 U pk | 재고문의 | pk | 595,000원 | 654,500원 |
다른 상품 둘러보기
Recombinant RNase Inhibitor ver.2.0 is an oxidation-resistant RNase inhibitor that is highly stable in enzymatic reactions requiring low DTT concentrations, such as in vitro transcription, in vitro translation, or RT-PCR. Derived from porcine liver, Recombinant RNase Inhibitor ver.2.0 displays enhanced oxidation resistance due to a mutation in a cysteine residue that is very sensitive to oxidation, which prevents degradation and inactivation of the enzyme under oxidizing reaction conditions.
Recombinant RNase Inhibitor ver.2.0 is an oxidation-resistant RNase inhibitor that is highly stable in enzymatic reactions requiring low DTT concentrations, such as in vitro transcription, in vitro translation, or RT-PCR. Derived from porcine liver, Recombinant RNase Inhibitor ver.2.0 displays greatly enhanced oxidation resistance due to a mutation in a cysteine residue that is very sensitive to oxidation, which prevents degradation and inactivation of the enzyme under oxidizing reaction conditions.
The enzyme forms a 1:1 complex specifically with RNase A to inhibit RNase activity; however, it is not effective against RNase H activity. The inhibitory reaction is reversible, and the inhibitor can be irreversibly inactivated to restore ribonuclease activity by dissociating the complex with urea or a sulfhydryl reagent. Moreover, unlike other non-protein competitive inhibitors (e.g., nucleotides and inorganic phosphates), it can easily be removed from the reaction system by phenol extraction.
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