Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4)
인간 CD4 항원을 인식하는 마우스 단클론 항체로, IHC, Flow Cytometry 등에 사용 가능. 비결합형(UNCONJUGATED) 형태로 제공되며, 단백질 A 정제 및 PBS 완충액에 0.09% sodium azide 포함. CD4 발현 연구 및 T세포 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:100 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | 10 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T4 |
| Immunogen | Human PHA blasts |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538413 |
Available Conjugated Formats:
Product Specific Information
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing.
Acetone fixation is recommended for frozen sections.
Suggested positive control for IHC: human lymphatic tissue.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and functions as a co-receptor for HIV.
CD4 is mainly expressed on T helper cells but can also be found on monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
CD4 participates in early T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck.
It may also contribute to neuronal damage in infectious and immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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