Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4)

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats:

• FITC
• PE
• Request custom conjugation

Product Specific Information

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing.
Acetone fixation is recommended for frozen sections.
Suggested positive control for IHC: human lymphatic tissue.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and functions as a co-receptor for HIV.
CD4 is mainly expressed on T helper cells but can also be found on monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
CD4 participates in early T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck.
It may also contribute to neuronal damage in infectious and immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.