Thermo Fisher Scientific Phospho-Paxillin (Tyr118) Monoclonal Antibody (GT162)
Phospho-Paxillin (Tyr118) 단일클론 항체(GT162)는 인간 Paxillin의 인산화 Tyr118 부위를 인식하는 Mouse IgG2b 항체입니다. Western blot과 ICC/IF에 적합하며, Protein A로 정제된 액상 제품입니다. 단기 4°C, 장기 -20°C 보관을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100 – 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT162 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Paxillin (phospho Tyr118). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.15 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735316 |
Product Specific Information
- Positive Control: A431 (1 mM pervanadate, 37°C, 30 min), HeLa
- Predicted Reactivity: Mouse (100%), Rat (100%), Chicken (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain, independently of the extracellular matrix. Although initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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