Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, Biotin
Rabbit 유래 anti-Human IgG (H+L) 2차 항체로 Biotin 결합형 제품. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용 가능. 높은 특이성과 감도를 보장하며, 연구용으로만 사용 가능.
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Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535682 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- [AP Conjugate]
- [FITC Conjugate]
- [HRP Conjugate]
- [Texas Red Conjugate]
- [TRITC Conjugate]
- [Custom Conjugation Request]
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, allowing use with multiple detection systems (e.g., HRP, AP, fluorescence). They also enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Notes
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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