Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, FITC
Rabbit anti-Human IgG (H+L) FITC 결합 2차 항체로, 면역세포염색 및 유세포분석에 적합합니다. 인간 IgG에 특이적으로 반응하며, 높은 감도와 신뢰성을 제공합니다. 친화 크로마토그래피로 정제되어 낮은 비특이적 결합을 보장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535679 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP conjugate
- Biotin conjugate
- HRP conjugate
- Texas Red conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is tested for sensitivity by ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit high specificity for human immunoglobulins. They are widely used for detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These reagents are typically generated by immunizing host animals with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for improved performance.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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