Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, AP
Rabbit 유래 Human IgG (H+L) 검출용 AP 결합 2차 항체. Western blot, IHC, ELISA에 사용 가능. 높은 특이성과 민감도 제공. 친화 크로마토그래피로 정제된 액상 형태. 연구용으로만 사용.
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Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535681 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is validated by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are used for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to achieve high specificity.
Additional Formats
- Biotin
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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