Thermo Fisher Scientific CD53 Recombinant Rabbit Monoclonal Antibody (004)
Thermo Fisher Scientific의 CD53 Recombinant Rabbit Monoclonal Antibody (Clone 004)는 Mouse CD53 단백질에 특이적으로 반응하는 항체로, Western blot 및 Immunoprecipitation에 적합합니다. 재조합 기술로 높은 특이성과 재현성을 제공하며, 방부제 무첨가 PBS 용액 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 0.5–2 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 004 |
| Immunogen | Recombinant Mouse CD53 Protein (Glu107–Asn181) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785503 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05–0.1%) to prevent contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Mouse CD53.
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid–myeloid lineage. It forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, or tetraspanins CD37, CD81, and CD82, modulating various signaling processes.
CD53 is involved in radioresistance of tumor cells and has anti-apoptotic effects upon activation. In the thymus, CD53 is upregulated during T cell positive selection and strongly expressed in macrophages exposed to bacterial lipopolysaccharide, whereas neutrophil stimulation leads to downregulation of CD53 expression.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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