Thermo Fisher Scientific beta Actin Polyclonal Antibody
Western blot, ICC/IF, IP 등 다양한 응용에 적합한 베타 액틴 폴리클로날 항체. 인간, 마우스, 랫트 등 다양한 종에 반응. 단백질 구조 및 세포 골격 연구에 유용하며, Western blot 로딩 컨트롤로 널리 사용됨.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 55 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 2 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | – | View 1 publication |
| Immunoprecipitation (IP) | 2 µg | View 1 publication |
| Miscellaneous (PubMed) | – | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Gerbil, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified full-length recombinant human beta-actin expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 30% glycerol, 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539914 |
Product Specific Information
- By Western blot, PA1-183 detects a prominent ~42 kDa protein in human, mouse, non-human primate, and rat whole cell lysates.
- For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all human cell types, involved in cell motility, structure, and integrity.
Beta and gamma actin coexist in most cell types as components of the cytoskeleton. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin), a two-stranded helix structure.
Because beta actin is ubiquitously expressed, it is widely used as a loading control in protein detection assays such as Western blotting.
Antibodies to beta-actin are valuable tools for studying intracellular actin distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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