Thermo Fisher Scientific SARS-CoV-2 Spike Protein (S1/S2) (aa16-1213), His-Flag Tag Recombinant Protein
SARS-CoV-2 Spike Protein(S1/S2) 재조합 단백질로, Insect cell에서 발현된 His-Flag 태그 단백질입니다. ELISA, Western blot 등 다양한 연구용에 사용 가능하며, 90% 이상 순도와 높은 안정성을 제공합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | Insect cells |
| Amino Acid Sequence | SARS-CoV-2 Spike Protein S1+S2, full length (s-ECD region) with a His-tag and a FLAG-tag |
| Tag | DYKDDDDK-tag, His-tag |
| Molecular Weight | 135 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >90% by SDS-PAGE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.3 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.2, with 10% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
- Source: The protein was purified from SF21 cells.
- Target: The S-ECD target covers S1 and S2.
- Purity: >95% by SDS-PAGE.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses ranging from mild cold-like symptoms to severe respiratory distress.
The CoV Spike (S) protein is essential for viral attachment, fusion, and entry, making it a key target for antibody, inhibitor, and vaccine development.
The receptor-binding domain (RBD, S-RBD) within the Spike protein binds strongly to human and bat ACE2 receptors, with SARS-CoV-2 RBD showing higher affinity than SARS-CoV RBD.
S1 contains the RBD, responsible for binding to ACE2, while S2 mediates membrane fusion.
The full-length Spike protein (1273 amino acids) includes a signal peptide (aa 1–13), S1 subunit (aa 14–685), and S2 subunit (aa 686–1273).
The S-ECD corresponds to the extracellular domain of the Spike protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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