Thermo Fisher Scientific TCF4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCF4,uniProtId:P15884-1,ncbiNodeId:9606,antigenRange:500-600,antigenLength:667,antigenImageFileName:PA5-77796_TCF4_P15884-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77796_TCF4_P15884-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2736688

Product Specific Information

A 1:1,000 dilution of PA5-77796 was sufficient for detection of TCF4 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects 71 kDa, 45 kDa. 45 kDa band represents a different isoform .

Target Information

TCF4 is transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box (E-box) binding site (CANNTG)-a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Defects in this gene are a cause of Pitt-Hopkins syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.