Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Functional Grade, eBioscience
마우스 CD3e를 인식하는 145-2C11 클론 단클론 항체로 T세포 표지 및 활성화 연구에 적합. Flow cytometry 및 기능적 분석에 사용 가능. 1 mg/mL 농도의 액상 형태로 제공되며 4°C에서 보관. Endotoxin 수준이 매우 낮아 세포 실험에 안전.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 4 |
| Immunohistochemistry (IHC) | - | 6 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 3 |
| Flow Cytometry (Flow) | 0.5 µg/test | 102 |
| ELISA | - | 4 |
| ChIP assay (ChIP) | - | 1 |
| Functional Assay | Assay-Dependent | 90 |
| Blocking Assay (BLOCK) | - | 2 |
| T-Cell Activation (TCA) | - | 5 |
| In vitro Assay (IV) | - | 8 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 145-2C11 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865573 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV Series (496, 563, 615, 661, 737)
- Brilliant Violet Series (421, 480, 605, 650, 711, 786)
- eFluor 450
- FITC
- NovaFluor Series (Blue, Red, Yellow variants)
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright Series (436, 600, 780)
- Request custom conjugation
Product Specific Information
Description:
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed on thymocytes and mature T cells. Binding of 145-2C11 to TCR induces intracellular signaling that can lead to activation, proliferation, or apoptosis depending on conditions. It is widely used as a phenotypic marker for mouse T cells.
Applications Reported:
- Flow cytometric analysis
- Cell activation
- Cell depletion
For in vitro T-cell activation protocols, see BestProtocols®.
Applications Tested:
Tested by flow cytometry using mouse splenocytes. Recommended ≤0.5 µg/test (in 100 µL final volume). Optimal antibody concentration should be titrated for each assay.
Storage and Handling:
Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered
Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)
Target Information
The CD3 complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, all encoded on human chromosome 11. These subunits associate with cytoplasmic signaling molecules via tyrosine-based motifs. CD3 plays a role in TCR-induced growth arrest, survival, and proliferation. It is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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