Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), Super Bright 600, eBioscience

Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.03 µg)/test
  View 3 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

This 9E2 monoclonal antibody reacts with human CD335 (NKp46), a member of the natural cytotoxicity receptor family (NKp30, NKp44). CD335 is expressed on NK cells and plays a role in activation and expansion of resting NK cells upon interaction with dendritic cells, facilitating elimination of tumor and virus-infected cells (e.g., influenza). It also interacts with pancreatic beta cells in type I diabetes development.

• Reported Applications: Flow cytometric analysis
• Tested Applications: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
  Use at 5 µL (0.03 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Super Bright 600 is a tandem dye excited by the violet laser line (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 2–8°C with minimal impact on brightness and FRET efficiency.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to enhanced NK cell-mediated tumor cell lysis. It consists of two Ig-like domains assembled with leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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