Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Super Bright 600, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 13 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an ITIM motif and plays a key role in peripheral tolerance and autoimmune regulation. It is expressed mainly on activated T and B lymphocytes. PD-L1 (B7-H1) and PD-L2 (B7-DC) are known ligands of PD-1 and are upregulated on activated antigen-presenting cells.

Costaining experiments indicate that eBioJ105 recognizes a different epitope than MIH4 (Product # 11-9969).

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested for flow cytometry using stimulated human peripheral blood cells
  • Recommended use: 5 µL (0.5 µg) per test
  • Test volume: 100 µL
  • Cell number: 10⁵–10⁸ cells/test

Super Bright 600 dye:

• Excitation: 405 nm
• Emission: 600 nm
• Recommended filter: 610/20 bandpass
• Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes to minimize non-specific interactions.
• Light sensitive: protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

T-cell activation triggers immune responses through recognition of peptide-MHC complexes on antigen-presenting cells (APCs). PD-1 (also known as PDCD-1) acts as an inhibitory receptor that binds to PD-L1 and PD-L2, downregulating T-cell activation in the absence of danger signals. PD-1 deficiency leads to hyperactivated immune responses and autoimmune phenotypes. Although PD-1 has a predicted molecular weight of 55 kDa, it can migrate at higher molecular weights in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지