Thermo Fisher Scientific WIT Wishful Thinking Polyclonal Antibody, FITC
FITC 결합 형광 표지 폴리클로날 항체로 Drosophila Wishful Thinking 단백질 검출에 사용. WB, IHC, ICC, ELISA 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제. -20°C 암소 보관.
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Thermo Fisher Scientific WIT Wishful Thinking Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 700–750 of WIT Wishful Thinking (D. melanogaster) protein |
| Conjugate | FITC (Fluorescein) |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Wishful thinking (wit) gene is the Drosophila homolog of the vertebrate BMP type II receptor. Mutations in wit result in pharate lethality that can be rescued by expression of a wit transgene in motor neurons but not in muscles. Mutant larvae exhibit small synapses, severe defects in evoked junctional potentials, lower frequency of spontaneous vesicle release, and altered synaptic active zone ultrastructure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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