
Thermo Fisher Scientific WIT Wishful Thinking Polyclonal Antibody, Biotin
Fruit fly 반응성의 Rabbit Polyclonal 항체로 Biotin 결합형. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. Affinity chromatography로 정제되어 높은 특이성과 안정성 제공. 연구용으로 Drosophila BMP type II receptor 분석에 적합.
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Thermo Fisher Scientific WIT Wishful Thinking Polyclonal Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 700–750 on WIT Wishful Thinking D. melanogaster protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Wishful thinking (wit) gene is the Drosophila homolog of the vertebrate BMP type II receptor. Mutations in wit cause pharate lethality that can be rescued by expression of a wit transgene in motor neurons but not in muscles. Mutant larvae exhibit small synapses, severe defects in evoked junctional potentials, lower frequency of spontaneous vesicle release, and altered ultrastructure of synaptic active zones.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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