
Thermo Fisher Scientific WIT Wishful Thinking Polyclonal Antibody
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Fruit fly(Wishful Thinking) 단백질을 인식하는 Rabbit Polyclonal Antibody. WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Affinity chromatography로 정제된 고순도 항체. 연구용으로만 사용 가능.
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Thermo Fisher Scientific WIT Wishful Thinking Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 700–750 on WIT Wishful Thinking D. melanogaster protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Wishful thinking (wit) gene is the Drosophila homolog of the vertebrate BMP type II receptor.
Mutations in wit result in pharate lethality, which can be rescued by expression of a wit transgene in motor neurons but not in muscles.
Mutant larvae exhibit:
- Small synapses
- Severe defects in evoked junctional potentials
- Lower frequency of spontaneous vesicle release
- Altered ultrastructure of synaptic active zones
Additional Information
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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