Thermo Fisher Scientific AMPK alpha-1,2 Polyclonal Antibody
AMPK alpha-1,2 단백질의 내인성 수준을 검출하는 Thermo Fisher Scientific의 폴리클로날 항체. 인간, 생쥐, 랫트 반응성. Western blot, IHC, ICC/IF에 적합. 고순도 친화 크로마토그래피 정제. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PRKAA1 (Accession Q13131), corresponding to amino acid residues Y470–S520. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899483 |
Product Specific Information
Antibody detects endogenous levels of total AMPK alpha.
Target Information
The protein encoded by this gene is a catalytic subunit of AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It functions as an energy-sensing enzyme that monitors cellular energy status. Under metabolic stress, AMPK is activated and phosphorylates key enzymes such as acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), regulating fatty acid and cholesterol biosynthesis. Studies in mice suggest this subunit influences insulin sensitivity and myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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