Thermo Fisher Scientific Aes Polyclonal Antibody
AES 단백질을 인식하는 Rabbit Polyclonal Antibody로 WB, IHC, ICC/IF 등에 사용 가능. 인간, 생쥐, 랫트 반응성. Affinity chromatography로 정제된 고순도 항체이며, PBS/glycerol buffer에 보관. 연구용 전용 제품.
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Thermo Fisher Scientific AES Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AES (Accession Q08117), corresponding to amino acid residues M1–L43 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899482 |
Product Specific Information
This antibody detects endogenous levels of total Amino-terminal enhancer of split (AES).
Target Information
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. AES is a member of the Groucho/transducin-like enhancer of split (TLE) family of transcriptional regulators, which act as co-repressors in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein released into the cytoplasm upon onset of apoptosis. This complex suppresses survival-promoting gene transcription controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 can be inhibited if cells attach to fibronectin via alpha5beta1 integrin, suggesting that the Bit1–AES pathway contributing to anoikis is regulated by integrins, particularly alpha5beta1.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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