Thermo Fisher Scientific CYP27A1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 154 and 460 of Human CYP27A1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYP27A1,uniProtId:Q02318-1,ncbiNodeId:9606,antigenRange:154-460,antigenLength:531,antigenImageFileName:PA5-27946_CYP27A1_Q02318-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27946_CYP27A1_Q02318-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545422

Product Specific Information

Recommended positive controls: A549, mouse liver, rat liver.

Predicted reactivity: Pig (83%), Rabbit (83%), Rhesus Monkey (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways. [1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3beta-hydroxy-5-cholestenoic acid. It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.