Thermo Fisher Scientific Collagen I Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

-

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 6 publications 6 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

1:5,000-1:50,000

-

Immunoprecipitation (IP)

1:100

-

Dot blot (DB)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen I,uniProtId:P02452-1,ncbiNodeId:9606,antigenRange:1-1464,antigenLength:1464,antigenImageFileName:PA1-26204_Collagen_I_P02452-1_Rabbit.svg,antigenImageFileNamePDP:PA1-26204_Collagen_I_P02452-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Collagen I,uniProtId:P02453-1,ncbiNodeId:9913,antigenRange:1-1463,antigenLength:1463,antigenImageFileName:PA1-26204_Collagen_I_P02453-1_Rabbit.svg,antigenImageFileNamePDP:PA1-26204_Collagen_I_P02453-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Collagen I,uniProtId:P02465-1,ncbiNodeId:9913,antigenRange:1-1364,antigenLength:1364,antigenImageFileName:PA1-26204_Collagen_I_P02465-1_Rabbit.svg,antigenImageFileNamePDP:PA1-26204_Collagen_I_P02465-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:Collagen I,uniProtId:P08123-1,ncbiNodeId:9606,antigenRange:1-1366,antigenLength:1366,antigenImageFileName:PA1-26204_Collagen_I_P08123-1_Rabbit.svg,antigenImageFileNamePDP:PA1-26204_Collagen_I_P08123-1_Rabbit_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

20mM potassium phosphate, pH 8.0, with 150mM NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2260734

Product Specific Information

Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.