Thermo Fisher Scientific Collagen II Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:400

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:5,000-1:50,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Bovine, Human, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen type II from adult human knee cartilage and bovine nasal cartilage. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen II,uniProtId:P02458-2,ncbiNodeId:9606,antigenRange:1-1487,antigenLength:1487,antigenImageFileName:PA1-26206_Collagen_II_P02458-2_Rabbit.svg,antigenImageFileNamePDP:PA1-26206_Collagen_II_P02458-2_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Collagen II,uniProtId:P02459-1,ncbiNodeId:9913,antigenRange:1-1487,antigenLength:1487,antigenImageFileName:PA1-26206_Collagen_II_P02459-1_Rabbit.svg,antigenImageFileNamePDP:PA1-26206_Collagen_II_P02459-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.03 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.020M potassium phosphate, pH 8.0, with 0.150M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_779883

Product Specific Information

Recommended positive controls: chondrocyte tissue.

PA1-26206 is expected to cross react with most mammals due to sequence homology. This antibody shows negligable cross reactivity with the Collagens 1, 3, 4, 5, and 6.

Target Information

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.