Thermo Fisher Scientific DNMT1 Monoclonal Antibody (4G11-C7), PerCP
DNMT1 단백질을 인식하는 PerCP 결합 마우스 단클론 항체로, WB, IHC, IP, ChIP 등 다양한 응용에 적합함. 인간, 생쥐, 제브라피시 등에 반응하며, 고순도 Protein G 정제 및 보존제 무첨가. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | 1:1,000 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fish, Human, Mouse, Zebrafish |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G11-C7 |
| Immunogen | Partial protein corresponding to amino acids 620–950 of human DNMT1 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931750 |
Additional Formats
Product Specific Information
2 µg/mL of MA5-45296 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 180 kDa and cross-reacts with mouse DNMT1.
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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