Thermo Fisher Scientific CENPA Monoclonal Antibody (5A7-2E11), APC
Thermo Fisher Scientific의 CENPA 단클론 항체(APC 결합형)는 인간 CENP-A 단백질을 특이적으로 인식합니다. Western blot 및 면역세포화학(ICC/IF)에 적합하며, 단백질 G로 정제된 액상 형태로 제공됩니다. 형광 특성은 651/660 nm입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A7-2E11 |
| Immunogen | Synthetic peptide corresponding to a portion of human CENP-A |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931751 |
Additional Formats
- Unconjugated (Cat. No. MA5-27710)
- FITC (Cat. No. MA5-45298)
- PE (Cat. No. MA5-45300)
- PerCP (Cat. No. MA5-45299)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45297 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 18 kDa. Recognizes Human CENP-A.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein containing a histone H3-related histone fold domain required for targeting to the centromere. It is proposed to be part of a modified nucleosome structure replacing one or both copies of conventional histone H3 in the (H3-H4)₂ tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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