Thermo Fisher Scientific DNMT1 Monoclonal Antibody (4G11-C7), FITC
DNMT1 단백질 검출용 FITC 결합 단클론 항체로 Western blot, IHC, IP, ChIP에 사용 가능. 인간, 생쥐, 어류, 제브라피쉬 반응성. 단백질 G 정제, 1 mg/mL 농도, 4°C 암소 보관. 형광 최대치 498/517 nm.
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Thermo Fisher Scientific DNMT1 Monoclonal Antibody (4G11-C7), FITC
Applications 및 Tested Dilution
| Application | Tested Dilution | 참고 |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | 1:1,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fish, Human, Mouse, Zebrafish |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G11-C7 |
| Immunogen | Raised against a partial protein corresponding to amino acids 620–950 of human DNMT1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931749 |
Additional Formats
- APC conjugate: MA5-45294
- PerCP conjugate: MA5-45296
- Custom conjugation available upon request
Product Specific Information
- 2 µg/mL of MA5-45295 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 180 kDa.
- Cross-reacts with mouse DNMT1.
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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