Thermo Fisher Scientific CENPA Monoclonal Antibody (5A7-2E11), FITC
FITC로 표지된 CENPA 단클론 항체로 인간 CENP-A 단백질을 특이적으로 인식. Western blot 및 면역세포화학(ICC/IF)에 사용 가능. 단백질 G로 정제된 액상 항체로 4°C 암소 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A7-2E11 |
| Immunogen | Synthetic peptide corresponding to a portion of human CENP-A |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931752 |
Available Formats
- Unconjugated (MA5-27710)
- APC (MA5-45297)
- PE (MA5-45300)
- PerCP (MA5-45299)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45298 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 18 kDa. Recognizes Human CENP-A.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein containing a histone H3-related histone fold domain required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces one or both copies of conventional histone H3 in the (H3-H4)₂ tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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