Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11)
MEK2 단백질의 C-말단을 인식하는 Mouse monoclonal 항체로, Western blot 및 ELISA에 적합합니다. Human, Mouse, Rat에 반응하며 MEK1과는 교차 반응하지 않습니다. Protein A로 정제된 액상 형태로, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12A6.G1.G11 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus of MEK2 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Biotin
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at -20°C prior to opening. This product is stable at 4°C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
This antibody reacts specifically with human MEK2. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction. MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop. MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, providing negative feedback regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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