Thermo Fisher Scientific IDO Recombinant Rabbit Monoclonal Antibody (7H8L17)
IDO1 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공합니다. 인간 반응성이며 원숭이, 돼지, 말, 염소에도 반응이 예측됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H8L17 |
| Immunogen | Protein corresponding to human IDO (aa1–aa403) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2716890 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Horse, and Goat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of key regulatory molecules such as tryptophan, serotonin, and melatonin. This reaction initiates the production of biologically active metabolites known as kynurenines.
IDO1 is expressed in various human tissues, macrophages, and dendritic cells (DCs). During inflammation, interferons (IFNs) induce IDO1 expression, contributing to antimicrobial defense by degrading tryptophan and inhibiting the growth of viruses, bacteria, and parasites. Additionally, IDO1 plays a crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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