Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT412)
HMGB1 단백질을 검출하기 위한 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트에 반응. Protein G 정제, PBS 버퍼, 무방부제. 염증 및 세포 신호 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT412 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.79 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787104 |
Product Specific Information
- Keep as a concentrated solution.
- Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).
- Positive Control: 293T, A431, HeLa, HepG2, Mouse brain, Rat brain.
- Store product as a concentrated solution and centrifuge briefly prior to opening the vial.
Target Information
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. Recent studies have shown that damaged or necrotic cells release HMGB1 into the extracellular space, where it acts as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide comprising three domains:
- Two positively charged DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
- A negatively charged 30 aa C-terminal acidic tail region
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The B box domain contains cytokine activity, while the A box interacts with transcription factors.
HMGB1 functions as both an inflammatory mediator and a regulator of T cell–dendritic cell interactions. It signals through receptors such as RAGE, TLR2, and TLR4.
HMGB1 is highly conserved and ubiquitously expressed in nuclei and cytoplasm of most cell types. It is a key mediator of inflammation in both sterile and infection-associated conditions. Elevated HMGB1 levels are observed during tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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