Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT412)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Keep as a concentrated solution.
• Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).
• Positive Control: 293T, A431, HeLa, HepG2, Mouse brain, Rat brain.
• Store product as a concentrated solution and centrifuge briefly prior to opening the vial.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. Recent studies have shown that damaged or necrotic cells release HMGB1 into the extracellular space, where it acts as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide comprising three domains:

• Two positively charged DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
• A negatively charged 30 aa C-terminal acidic tail region

Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The B box domain contains cytokine activity, while the A box interacts with transcription factors.
HMGB1 functions as both an inflammatory mediator and a regulator of T cell–dendritic cell interactions. It signals through receptors such as RAGE, TLR2, and TLR4.

HMGB1 is highly conserved and ubiquitously expressed in nuclei and cytoplasm of most cell types. It is a key mediator of inflammation in both sterile and infection-associated conditions. Elevated HMGB1 levels are observed during tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.