Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT349)
HMGB1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체(GT349). Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며 인간, 마우스, 랫트 등에서 반응. 고농축 액상 형태로 제공되며 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT349)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT349 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMGB1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787105 |
Product Specific Information
- Keep as concentrated solution.
- Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)
- Positive Control: 293T, A431, HeLa, HepG2, Mouse brain, Rat brain
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein. Recent studies indicate that damaged, necrotic cells release HMGB1 into the extracellular milieu, where it functions as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:
- Two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162)
- A negatively charged 30 aa C-terminal acidic tail region
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
Cytokine activity resides in the B box, while the A box interacts with transcription factor domains.
HMGB1 acts as:
- An inflammatory mediator promoting monocyte migration and cytokine secretion
- A mediator of T cell–dendritic cell interaction
- A signaling molecule via receptors RAGE, TLR2, and TLR4
It is highly conserved and ubiquitous in nuclei and cytoplasm, serving as a key mediator of inflammation during sterile and infection-associated responses. HMGB1 levels are elevated during tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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