Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT349)

Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT349)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)
• Positive Control: 293T, A431, HeLa, HepG2, Mouse brain, Rat brain
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein. Recent studies indicate that damaged, necrotic cells release HMGB1 into the extracellular milieu, where it functions as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:

• Two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162)
• A negatively charged 30 aa C-terminal acidic tail region

Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
Cytokine activity resides in the B box, while the A box interacts with transcription factor domains.

HMGB1 acts as:

• An inflammatory mediator promoting monocyte migration and cytokine secretion
• A mediator of T cell–dendritic cell interaction
• A signaling molecule via receptors RAGE, TLR2, and TLR4

It is highly conserved and ubiquitous in nuclei and cytoplasm, serving as a key mediator of inflammation during sterile and infection-associated responses. HMGB1 levels are elevated during tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.