Thermo Fisher Scientific 5-Methylcytosine Monoclonal Antibody (GT4111)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunohistochemistry - Free Floating (IHC (Free))

1:50-1:500

-

Dot blot (DB)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Methylated DNA Immunoprecipitation (MeDIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT4111

Immunogen

The immunogen used to generate this antibody corresponds to 5-Methylcytosine/5-mC.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.7 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787107

Product Specific Information

Keep as concentrated solution.

Positive Control: SH-SY5Y genomic DNA.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.