Thermo Fisher Scientific CDCA8 Monoclonal Antibody (OTI7G7), TrueMAB

Applications

• Western Blot (WB): 1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CDCA8 is a component of the chromosomal passenger complex (CPC), essential for the stability of the bipolar mitotic spindle.
The CPC, composed of Survivin, CDCA8, INCENP, and Aurora-B, plays a crucial role during mitosis and cell division.
Expression of CDCA8 increases during the G2/M phase and decreases after mitotic exit. It is cell cycle-regulated, down-regulated by p53/Rb signaling, and up-regulated in various cancers.
Inactivation of CDCA8 in Drosophila results in polyploidy, delayed mitosis, and abnormal tissue development, highlighting its role in cell proliferation.
Aberrant CDCA8 expression is associated with poor prognosis in gastric cancer and is essential for early embryonic development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.