Thermo Fisher Scientific IL-3 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-3. Anti-Human IL-3-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIL-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-3 (500-P23) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIL-3.

Western Blot:
To detect hIL-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-3 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P23BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Most of these functions are enhanced or dependent on co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity at the amino acid level, and the bioactivity of IL-3 is highly species specific.
Human IL-3 has no activity on mouse cells.
IL-3 is a potent growth-promoting cytokine capable of supporting proliferation of various hematopoietic cell types and is involved in cell growth, differentiation, and apoptosis.
It may also possess neurotrophic activity and be associated with neurologic disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.