Thermo Fisher Scientific beta-3 Defensin Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 4 publications 4 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930000

Product Specific Information

AA Sequence of recombinant protein: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-3. Anti-Human BD-3-specific antibody was purified by affinity chromatography employing an immobilized Human BD-3 matrix.

Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-3 (500-P241Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3.

Western Blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Oct 2014].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.