Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 42 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 is found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and plays an important role in T cell–B cell interaction and effector cell maturation. LG.7F9 cross-reacts with human and rat CD27.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes. ≤0.125 µg per test (100 µL volume, 10⁵–10⁸ cells/test).
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily (includes CD40, CD30). It regulates cell proliferation and death, with expression mainly on lymphocytes. CD27 forms disulfide-linked homodimers on mature thymocytes, peripheral T cells, and some B cells. Activation via TCR-CD3 upregulates CD27 and releases soluble CD27 (sCD27), a prognostic marker in B cell malignancies. CD27 interacts with CD70 and SIVA, influencing apoptosis and immune regulation. Dysfunctions in CD27 are linked to Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures.

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