Thermo Fisher Scientific CD25 Monoclonal Antibody (BC96), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 0.25 µg/test
  View 57 publications

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 532, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 615, 737, 805
• Brilliant Violet 421, 711
• eFluor 450
• FITC
• NovaFluor Red 685, Red 700, Yellow 700
• PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα).
CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 with low affinity, but associates with CD122 (IL-2Rβ) and CD132 (common γ chain) to form the high-affinity IL-2 receptor complex.
CD25 plays a role in lymphocyte differentiation, activation, and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: ≤ 0.25 µg/test (in 100 µL volume; 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD25 (IL2RA) encodes the alpha chain of the interleukin-2 receptor.
It forms a high-affinity receptor complex with IL2RB and IL2RG, mediating IL-2 signaling crucial for T and B lymphocyte proliferation.
IL2RA expression is tightly regulated and monoallelic in mature thymocytes.
Mutations can lead to immune dysregulation such as ulcerative colitis-like disease, highlighting its role in immune homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지