Thermo Fisher Scientific ATG3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:300–1:2,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:300–1:2,000

Miscellaneous PubMed (Misc)

• Publications: View 3 publications

Product Specifications

Product Specific Information

• Reconstitute in 150 µL of sterile water.
• Centrifuge to remove any insoluble material.
• Specificity: ATG3.

Target Information

ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It helps preserve cellular nutrients under starvation conditions and supports normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 acts as an E2-like enzyme during autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, critical for autophagy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.